RT Journal Article SR Electronic T1 In Silico design and characterization of multiepitopes vaccine for SARS-CoV2 from its Spike proteins JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.06.03.131755 DO 10.1101/2020.06.03.131755 A1 Gunderao H Kathwate YR 2020 UL http://biorxiv.org/content/early/2020/06/03/2020.06.03.131755.abstract AB COVID 19 is disease caused by novel corona virus, SARS-CoV2 originated in China most probably of Bat origin. Till date there is no specific vaccine or drug has been discovered to tackle the infection. In response to this pandemic, we utilized bioinformatics knowledge to develop efficient vaccine candidate against SARS-CoV2. Designed vaccine is rich in effective BCR and TCR epitopes screened from the sequence of S-protein of SARS-CoV2. Bioinformatics tools were utilized for prediction, refinement and validation of tertiary structure of designed vaccine. Protein-Protein interaction prediction of TLR2/4 and designed vaccine indicate effective binding. Designed multiepitopes vaccine has induce Cell mediated and humoral immunity along with increased interferon gamma response. Macrophages and dendritic cells were also found increased over a vaccine exposure. In silico codon optimization and cloning in expression vector indicate that vaccine can be efficiently expressed in E. coli. In conclusion, predicted vaccine was good antigen probable no allergen and effectively induce cellular and humoral immunity.Competing Interest StatementThe authors have declared no competing interest.