RT Journal Article
SR Electronic
T1 Optical Sectioning of Live Mammal with Near-Infrared Light Sheet
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 447433
DO 10.1101/447433
A1 Feifei Wang
A1 Hao Wan
A1 Jingying Yue
A1 Mingxi Zhang
A1 Zhuoran Ma
A1 Qinchao Sun
A1 Liangqiong Qu
A1 Huilong Ma
A1 Yeteng Zhong
A1 Ye Tian
A1 Guosong Hong
A1 Wen Jung Li
A1 Yongye Liang
A1 Lianqing Liu
A1 Hongjie Dai
YR 2018
UL http://biorxiv.org/content/early/2018/10/18/447433.abstract
AB Deep-tissue three-dimensional optical imaging of live mammals in vivo with high spatiotemporal resolution in non-invasive manners has been challenging due to light scattering. Here, we developed near-infrared (NIR) light sheet microscopy (LSM) with optical excitation and emission wavelengths up to ~ 1320 nm and ~ 1700 nm respectively, far into the NIR-II (1000-1700 nm) region for 3D optical sectioning through live tissues. Suppressed scattering of both excitation and emission photons allowed one-photon optical sectioning at ~ 2 mm depth in highly scattering brain tissues. NIR-II LSM enabled non-invasive in vivo imaging of live mice, revealing never-before-seen dynamic processes such as highly abnormal tumor microcirculation, and 3D molecular imaging of an important immune checkpoint protein, programmed-death ligand 1 (PD-L1) receptors at the single cell scale in tumors. In vivo two-color near-infrared light sheet sectioning enabled simultaneous volumetric imaging of tumor vasculatures and PD-L1 proteins in live mammals.