PT - JOURNAL ARTICLE AU - Bo Zhou AU - Yiwu Yan AU - Yang Wang AU - Sungyong You AU - Michael R. Freeman AU - Wei Yang TI - Quantitative Proteomic Analysis of Prostate Tissue Specimens Identifies Deregulated Protein Complexes in Primary Prostate Cancer AID - 10.1101/447375 DP - 2018 Jan 01 TA - bioRxiv PG - 447375 4099 - http://biorxiv.org/content/early/2018/10/18/447375.short 4100 - http://biorxiv.org/content/early/2018/10/18/447375.full AB - Prostate cancer (PCa) is the most frequently diagnosed non-skin cancer and a leading cause of mortality among males in developed countries. However, our understanding of the global changes of protein complexes within PCa tissue specimens remains very limited, although it has been well recognized that protein complexes carry out essentially all major processes in living organisms and that their deregulation drives the pathogenesis and progression of various diseases. By coupling tandem mass tagging-synchronous precursor selection-mass spectrometry/mass spectrometry/mass spectrometry (TMT-SPSMS3) with differential expression and co-regulation analyses, the present study compared the differences between protein complexes in normal prostate, low-grade PCa, and high-grade PCa tissue specimens. Globally, a large downregulated putative protein-protein interaction (PPI) network was detected in both low-grade and high-grade PCa, yet a large upregulated putative PPI network was only detected in high-grade but not low-grade PCa, compared with normal controls. To identify specific protein complexes that are deregulated in PCa, quantified proteins were mapped to protein complexes in CORUM, a collection of experimentally verified mammalian protein complexes. Differential expression analysis suggested that mitochondrial ribosomes and the fibrillin-associated protein complex were significantly overexpressed, whereas the ITGA6-ITGB4-Laminin10/12 and the P2X7 receptor signaling complexes were significantly downregulated, in PCa compared with normal prostate. Moreover, differential co-regulation analysis indicated that the assembly levels of some nuclear protein complexes involved in RNA synthesis and processing were significantly increased in low-grade PCa, and those of mitochondrial complex I and its subcomplexes were significantly increased in high-grade PCa, compared with normal prostate. In summary, the study represents the first global and quantitative comparison of protein complexes in prostate tissue specimens. It is expected to enhance our understanding of the molecular mechanisms underlying PCa development and progression in human patients, as well as lead to the discovery of novel biomarkers and therapeutic targets for precision management of PCa.DEPDifferentially expressed proteinsFASPFilter-aided sample preparationFDRFalse discovery rateFFPEFormalin-fixed and paraffin-embeddedHGHigh-gradeIPAIngenuity Pathway AnalysisLCLiquid chromatographyLGLow-gradeMALDIMatrix-assisted laser desorption/ionizationMSMass spectrometryNNormalOCTOptimal cutting temperaturePCaProstate cancerPPIProtein-protein interactionSPSSynchronous precursor selection2DETwo-dimensional electrophoresisTMTtandem mass tag