RT Journal Article
SR Electronic
T1 Enhancing Colorimetric LAMP Amplification Speed and Sensitivity with Guanidine Chloride
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.06.03.132894
DO 10.1101/2020.06.03.132894
A1 Yinhua Zhang
A1 Guoping Ren
A1 Jackson Buss
A1 Andrew J. Barry
A1 Gregory C. Patton
A1 Nathan A. Tanner
YR 2020
UL http://biorxiv.org/content/early/2020/06/04/2020.06.03.132894.abstract
AB Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. The global SARS-CoV-2 pandemic has presented an urgent need for new and better diagnostic methods, with colorimetric LAMP utilized in numerous studies for SARS-CoV-2 detection. However, the sensitivity of colorimetric LAMP in early reports has been below that of the standard RT-qPCR tests, and we sought to improve performance. Here we report the use of guanidine hydrochloride and combined primer sets to increase speed and sensitivity in colorimetric LAMP, bringing this simple method up to the standards of sophisticated technique and enabling accurate and high-throughput diagnostics.Competing Interest StatementThe authors are employees of New England Biolabs, manufacturer of reagents described in the manuscript.