RT Journal Article
SR Electronic
T1 Large scale metagenome assembly reveals novel animal-associated microbial genomes, biosynthetic gene clusters, and other genetic diversity
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.06.05.135962
DO 10.1101/2020.06.05.135962
A1 Nicholas D. Youngblut
A1 Jacobo de la Cuesta-Zuluaga
A1 Georg H. Reischer
A1 Silke Dauser
A1 Nathalie Schuster
A1 Chris Walzer
A1 Gabrielle Stalder
A1 Andreas H. Farnleitner
A1 Ruth E. Ley
YR 2020
UL http://biorxiv.org/content/early/2020/06/05/2020.06.05.135962.abstract
AB Large-scale metagenome assemblies of human microbiomes have produced a vast catalogue of previously unseen microbial genomes; however, comparatively few microbial genomes derive from other vertebrates. Here, we generated 4374 metagenome assembled genomes (MAGs) from gut samples of 180 predominantly wild animal species representing 5 classes. Combined with existing datasets, we produced 5596 non-redundant, quality MAGs and 1522 species-level genome bins (SGBs). Most SGBs were novel at the species, genus, or family levels, and the majority were enriched in host versus environment metagenomes. Many traits distinguished SGBs enriched in host or environmental biomes, including the number of antimicrobial resistance genes. We identified 1986 diverse and largely novel biosynthetic gene clusters. Gene-based assembly revealed tremendous gene diversity, much of it host or environment specific. Our MAG and gene datasets greatly expand the microbial genome repertoire and provide a broad view of microbial adaptations to life within a living host.Competing Interest StatementThe authors have declared no competing interest.