RT Journal Article
SR Electronic
T1 ExM-STORM: Expansion Single Molecule Nanoscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 049403
DO 10.1101/049403
A1 Zhisong Tong
A1 Paolo Beuzer
A1 Qing Ye
A1 Josh Axelrod
A1 Zhenmin Hong
A1 Hu Cang
YR 2016
UL http://biorxiv.org/content/early/2016/04/20/049403.abstract
AB Recently, Chen et al. invented Expansion Microscopy, or ExM, which imprints a specimen’s features of interest onto a polymer hydrogel network and expands the network homogeneously to magnify sub-diffraction-limit features. However, ExM bleaches massively, 50-100% of the fluorescent molecules; thus it is not suited for single-molecule imaging. Here, we describe improved ExM that is free of bleaching, and thus can be synergically integrated with single-molecule nanoscopy, STORM.