TY - JOUR T1 - Measuring Neuronal Signals with Microelectrode Arrays: A Finite Element Analysis JF - bioRxiv DO - 10.1101/2020.06.07.139014 SP - 2020.06.07.139014 AU - R Bestel AU - U van Rienen AU - C Thielemann AU - R Appali Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/06/08/2020.06.07.139014.abstract N2 - Objective Measuring neuronal cell activity using microelectrode arrays reveals a great variety of derived signal shapes within extracellular recordings. However, possible mechanisms responsible for this variety have not yet been entirely determined, which might hamper any subsequent analysis of the recorded neuronal data. For an investigation of this issue, we propose a computational model based on the finite element method describing the electrical coupling between an electrically active neuron and an extracellular recording electrode in detail. This allows for a systematic study of possible parameters that may play an essential role in defining or altering the shape of the measured electrode potential. Our results indicate that neuronal geometry and neurite structure, as well as the actual pathways of input potentials that evoke action potential generation, have a significant impact on the shape of the resulting extracellular electrode recording and explain most of the known signal shape variety.Competing Interest StatementThe authors have declared no competing interest. ER -