PT  - JOURNAL ARTICLE
AU  - R Bestel
AU  - U van Rienen
AU  - C Thielemann
AU  - R Appali
TI  - Measuring Neuronal Signals with Microelectrode Arrays: A Finite Element Analysis
AID  - 10.1101/2020.06.07.139014
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.06.07.139014
4099  - http://biorxiv.org/content/early/2020/06/08/2020.06.07.139014.short
4100  - http://biorxiv.org/content/early/2020/06/08/2020.06.07.139014.full
AB  - Objective Measuring neuronal cell activity using microelectrode arrays reveals a great variety of derived signal shapes within extracellular recordings. However, possible mechanisms responsible for this variety have not yet been entirely determined, which might hamper any subsequent analysis of the recorded neuronal data. For an investigation of this issue, we propose a computational model based on the finite element method describing the electrical coupling between an electrically active neuron and an extracellular recording electrode in detail. This allows for a systematic study of possible parameters that may play an essential role in defining or altering the shape of the measured electrode potential. Our results indicate that neuronal geometry and neurite structure, as well as the actual pathways of input potentials that evoke action potential generation, have a significant impact on the shape of the resulting extracellular electrode recording and explain most of the known signal shape variety.Competing Interest StatementThe authors have declared no competing interest.