RT Journal Article
SR Electronic
T1 DIFFERENTIAL BIOENERGETICS IN ADULT RAT CARDIOMYOCYTES ISOLATED FROM THE RIGHT VERSUS LEFT VENTRICLE
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.06.08.133769
DO 10.1101/2020.06.08.133769
A1 Quyen L. Nguyen
A1 Krithika Rao
A1 Steven J. Mullett
A1 Stacy G. Wendell
A1 Claudette St. Croix
A1 Eric Goetzman
A1 Sruti Shiva
YR 2020
UL http://biorxiv.org/content/early/2020/06/09/2020.06.08.133769.abstract
AB The right and left ventricle of the heart have distinctly different developmental origins and are affected differently by similar pathological stimuli. Though it is well established that the heart relies almost entirely on mitochondrial function to sustain energy production, it remains unclear whether bioenergetics differ in the two ventricles. Herein, we define a novel methodology to optimize the isolation of intact cardiomyocytes from the right versus the left ventricle. We demonstrate that this segmental Langendorff-free methodology yields viable cardiomyocytes with intact mitochondrial function. Further, we compare bioenergetics in right versus left ventricle cardiomyocytes and show that cardiomyocytes from the right ventricle have a greater maximal capacity for respiration and enhanced glycolytic rate. This increase in respiration was concomitant with increased fatty acid oxidation and levels of fatty acid oxidation proteins, but no change in mitochondrial electron transport complex expression. These data validate a potentially powerful tool to evaluate differences in right and left ventricular function and advance the understanding of cardiac bioenergetic differences. These data will be discussed in the context of differential responses by the right versus ventricle in pathology.Competing Interest StatementThe authors have declared no competing interest.