TY - JOUR T1 - A set of functionally-defined brain regions with improved representation of the subcortex and cerebellum JF - bioRxiv DO - 10.1101/450452 SP - 450452 AU - Benjamin A. Seitzman AU - Caterina Gratton AU - Scott Marek AU - Ryan V. Raut AU - Nico U.F. Dosenbach AU - Bradley L. Schlaggar AU - Steven E. Petersen AU - Deanna J. Greene Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/10/24/450452.abstract N2 - An important aspect of network-based analysis is robust node definition. This issue is critical for functional brain network analyses, as poor node choice can lead to spurious findings and misleading inferences about functional brain organization. Two sets of functional brain nodes from our group are well represented in the literature: (1) 264 volumetric regions of interest (ROIs) reported in Power et al., 2011 and (2) 333 surface parcels reported in Gordon et al., 2016. However, subcortical and cerebellar structures are either incompletely captured or missing from these ROI sets. Therefore, properties of functional network organization involving the subcortex and cerebellum may be underappreciated thus far. Here, we apply a winner-take-all partitioning method to resting-state fMRI data and careful consideration of anatomy to generate novel functionally-constrained ROIs in the thalamus, basal ganglia, amygdala, hippocampus, and cerebellum. We validate these ROIs in three datasets via several anatomical and functional criteria, including known anatomical divisions and functions, as well as agreement with existing literature. Further, we demonstrate that combining these ROIs with established cortical ROIs recapitulates and extends previously described functional network organization. This new set of ROIs is made publicly available for general use, including a full list of MNI coordinates and functional network labels. ER -