%0 Journal Article %A Kamil Witek %A Xiao Lin %A Hari S Karki %A Florian Jupe %A Agnieszka I Witek %A Burkhard Steuernagel %A Remco Stam %A Cock van Oosterhout %A Sebastian Fairhead %A Jonathan M Cocker %A Shivani Bhanvadia %A William Barrett %A Chih-Hang Wu %A Hiroaki Adachi %A Tianqiao Song %A Sophien Kamoun %A Vivianne GAA Vleeshouwers %A Laurence Tomlinson %A Brande BH Wulff %A Jonathan DG Jones %T A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector %D 2020 %R 10.1101/2020.05.15.095497 %J bioRxiv %P 2020.05.15.095497 %X Late blight caused by Phytophthora infestans greatly constrains potato production. Many Resistance (R) genes were cloned from wild Solanum species and/or introduced into potato cultivars by breeding. However, individual R genes have been overcome by P. infestans evolution; durable resistance remains elusive. We positionally cloned a new R gene, Rpi-amr1, from Solanum americanum, that encodes an NRC helper-dependent CC-NLR protein. Rpi-amr1 confers resistance in potato to all 19 P. infestans isolates tested. Using association genomics and long-read RenSeq, we defined eight additional Rpi-amr1 alleles from different S. americanum and related species. Despite only ∼90% identity between Rpi-amr1 proteins, all confer late blight resistance but differentially recognize Avramr1 orthologs and paralogs. We propose that Rpi-amr1 gene family diversity facilitates detection of diverse paralogs and alleles of the recognized effector, enabling broad-spectrum and durable resistance against P. infestans.Competing Interest StatementKW, HSK, FGJ and JDGJ are named inventors on a patent application (PCT/US2017/066691) pertaining to Rpi-amr1 that was filed by the 2Blades Foundation on behalf of the Sainsbury Laboratory. %U https://www.biorxiv.org/content/biorxiv/early/2020/06/10/2020.05.15.095497.full.pdf