PT  - JOURNAL ARTICLE
AU  - Sergio Menchero
AU  - Antonio Lopez-Izquierdo
AU  - Isabel Rollan
AU  - Julio Sainz de Aja
AU  - Maria Jose Andreu
AU  - Minjung Kang
AU  - Javier Adan
AU  - Rui Benedito
AU  - Teresa Rayon
AU  - Anna-Katerina Hadjantonakis
AU  - Miguel Manzanares
TI  - Transitions in cell potency during early mouse development are driven by Notch
AID  - 10.1101/451922
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 451922
4099  - http://biorxiv.org/content/early/2018/10/24/451922.short
4100  - http://biorxiv.org/content/early/2018/10/24/451922.full
AB  - The Notch signalling pathway plays fundamental roles in diverse developmental processes in metazoans, where it is important in driving cell fate and directing differentiation of various cell types. However, we still have limited knowledge about the role of Notch in early preimplantation stages of mammalian development, or how it interacts with other signalling pathways active at these stages such as Hippo. By using genetic and pharmacological tools in vivo, together with image analysis of single embryos and pluripotent cell culture, we have found that Notch is active from the 4-cell stage. Transcriptomic analysis in single morula identified novel Notch targets, such as early naïve pluripotency markers or transcriptional repressors such as TLE4. Our results reveal a previously undescribed role for Notch in driving transitions during the gradual loss of potency that takes place in the early mouse embryo prior to the first lineage decisions.