PT  - JOURNAL ARTICLE
AU  - Lourds M. Fernando
AU  - Jeandele Elliot
AU  - Anna K. Allen
TI  - The <em>C. elegans</em> proteasome subunit RPN-12 is required for hermaphrodite germline sex determination and oocyte quality
AID  - 10.1101/2020.06.11.146472
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.06.11.146472
4099  - http://biorxiv.org/content/early/2020/06/15/2020.06.11.146472.short
4100  - http://biorxiv.org/content/early/2020/06/15/2020.06.11.146472.full
AB  - Background The proteasome is a multi-subunit complex and a major proteolytic machinery in cells. Most subunits are essential for proteasome function, and depletion of individual subunits normally results in lethality. RPN-12/Rpn12/PSMD8 is a lid subunit of the 19S regulatory particle (RP) of the 26S proteasome. Studies in Caenorhabditis elegans demonstrated that RNAi depletion of RPN-12 does not result in lethality. RPN-12 has not been well studied in higher eukaryotes. In this study we investigate the biological significance of RPN-12 in C. elegans.Results We found that the null mutant rpn-12(av93) did not cause major impairment of the proteolytic activity of the proteasome. Most rpn-12(av93) hermaphrodites lack sperm leading to feminization of the germ line that can be partially rescued by mating to males. The lack of sperm phenotype can be suppressed by downregulation of TRA-1, a player in the hermaphrodite germline sex determination pathway. Also, rpn-12(av93) animals show significant nuclear accumulation of the meiotic kinase WEE-1.3, a protein predominantly localized to the perinuclear region. Interestingly, chemical inhibition of the proteasome did not cause nuclear accumulation of WEE-1.3.Conclusions RPN-12 plays a previously unknown role in oogenesis and the germline sex determination pathway in C. elegans hermaphrodites.Competing Interest StatementThe authors have declared no competing interest.