PT  - JOURNAL ARTICLE
AU  - Ayako Kurimoto
AU  - Yuki Kawasaki
AU  - Toshiki Ueda
AU  - Tatsutoshi Inuzuka
TI  - Enhanced recovery of CD9-positive extracellular vesicles from human specimens by chelating reagent
AID  - 10.1101/2020.06.17.155861
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.06.17.155861
4099  - http://biorxiv.org/content/early/2020/06/18/2020.06.17.155861.short
4100  - http://biorxiv.org/content/early/2020/06/18/2020.06.17.155861.full
AB  - Extracellular vesicles (EVs) have gained attention as potential targets of early diagnostics and prognosis in the field of liquid biopsy. Despite clinical potentials, the best method to isolate EVs from specimens remains controversial due to low purity, low specificity, and lack of reproducibility with current isolation methods. Here we show that a chelating reagent enhances the recovery efficiency of EVs from crude biological samples by immunoprecipitation using an anti-CD9 antibody. Proteomic and western blotting analyses show that the EVs isolated using the chelating reagent contain a wider variety of proteins than those isolated with PBS.Competing Interest StatementThe authors have declared no competing interest.