RT Journal Article
SR Electronic
T1 Enhanced recovery of CD9-positive extracellular vesicles from human specimens by chelating reagent
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.06.17.155861
DO 10.1101/2020.06.17.155861
A1 Ayako Kurimoto
A1 Yuki Kawasaki
A1 Toshiki Ueda
A1 Tatsutoshi Inuzuka
YR 2020
UL http://biorxiv.org/content/early/2020/06/18/2020.06.17.155861.abstract
AB Extracellular vesicles (EVs) have gained attention as potential targets of early diagnostics and prognosis in the field of liquid biopsy. Despite clinical potentials, the best method to isolate EVs from specimens remains controversial due to low purity, low specificity, and lack of reproducibility with current isolation methods. Here we show that a chelating reagent enhances the recovery efficiency of EVs from crude biological samples by immunoprecipitation using an anti-CD9 antibody. Proteomic and western blotting analyses show that the EVs isolated using the chelating reagent contain a wider variety of proteins than those isolated with PBS.Competing Interest StatementThe authors have declared no competing interest.