RT Journal Article
SR Electronic
T1 Chromatin accessibility landscapes activated by cell surface and intracellular immune receptors
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.06.17.157040
DO 10.1101/2020.06.17.157040
A1 Pingtao Ding
A1 Toshiyuki Sakai
A1 Ram Krishna Shrestha
A1 Nicolas Manosalva Perez
A1 Wenbin Guo
A1 Bruno Pok Man Ngou
A1 Shengbo He
A1 Chang Liu
A1 Xiaoqi Feng
A1 Runxuan Zhang
A1 Klaas Vandepoele
A1 Dan MacLean
A1 Jonathan DG Jones
YR 2020
UL http://biorxiv.org/content/early/2020/06/18/2020.06.17.157040.abstract
AB Activation of cell Surface and Intracellular Receptor-Mediated Immunity (SRMI and IRMI) results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which SRMI and IRMI lead to transcriptional changes are not clear. Here, we combine RNA-seq and ATAC-seq to define changes in gene expression and chromatin accessibility; both SRMI and IRMI increase chromatin accessibility at induced defense genes. Analysis of ATAC-seq and RNA-seq data combined with publicly available information on transcription factor DNA-binding motifs enabled comparison of individual gene regulatory networks activated by SRMI and IRMI, and by both. These results and analyses reveal overlapping and conserved transcriptional regulatory mechanism between the two immune systems.Competing Interest StatementThe authors have declared no competing interest.