RT Journal Article
SR Electronic
T1 The role of trehalose 6-phosphate in shoot branching – local and non-local effects on axillary bud outgrowth in arabidopsis rosettes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.06.18.158568
DO 10.1101/2020.06.18.158568
A1 Franziska Fichtner
A1 Francois F. Barbier
A1 Maria G. Annunziata
A1 Regina Feil
A1 Justyna J. Olas
A1 Bernd Mueller-Roeber
A1 Mark Stitt
A1 Christine A. Beveridge
A1 John E. Lunn
YR 2020
UL http://biorxiv.org/content/early/2020/06/19/2020.06.18.158568.abstract
AB - Trehalose 6-phosphate (Tre6P) is a sucrose signalling metabolite that has been implicated in regulation of shoot branching, but its precise role is not understood.- We expressed tagged forms of TREHALOSE-6-PHOSPHATE SYNTHASE1 (TPS1) to determine where Tre6P is synthesized in arabidopsis (Arabidopsis thaliana), and investigated the impact of localized changes in Tre6P levels, in axillary buds or vascular tissues, on shoot branching in wild-type and branching mutant backgrounds.- TPS1 is expressed in axillary buds and the subtending vasculature, as well as in the leaf and stem vasculature. Expression of a heterologous trehalose-6-phosphate phosphatase (TPP) to lower Tre6P in axillary buds strongly delayed bud outgrowth in long days and inhibited branching in short days. TPP expression in the vasculature also delayed lateral bud outgrowth and decreased branching. Increased Tre6P in the vasculature enhanced branching and was accompanied by higher expression of FLOWERING LOCUS T (FT) and up-regulation of sucrose transporters. Increased vascular Tre6P levels enhanced branching in branched1 but not in ft mutant backgrounds.- These results provide direct genetic evidence of a local role for Tre6P in regulation of axillary bud outgrowth within the buds themselves, and also connect Tre6P with systemic regulation of shoot branching via FT.Competing Interest StatementThe authors have declared no competing interest.