PT - JOURNAL ARTICLE AU - Joshua Hutchings AU - Viktoriya G. Stancheva AU - Nick R. Brown AU - Alan M.C. Cheung AU - Elizabeth A. Miller AU - Giulia Zanetti TI - Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network AID - 10.1101/2020.06.18.159608 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.06.18.159608 4099 - http://biorxiv.org/content/early/2020/06/19/2020.06.18.159608.short 4100 - http://biorxiv.org/content/early/2020/06/19/2020.06.18.159608.full AB - The COPII coat mediates Endoplasmic Reticulum (ER) to Golgi trafficking of thousands of proteins. Five essential coat components assemble on the ER membrane to induce the formation of transport vesicles while selectively recruiting cargo proteins. Individual COPII proteins interact dynamically through multiple interfaces to assemble into inner and outer coat layers, forming coats of varied architectures. Here we have used cryo-electron tomography (cryo-ET) and subtomogram averaging to visualise the complete membrane-bound COPII coat to unprecedented detail, revealing a complex interaction network that includes a number of previously unknown interfaces. We identify essential interactions, and functionally characterise their contribution to COPII assembly and membrane remodelling. We find that multiple finely-tuned interfaces contribute to coat organization required to remodel membranes, depending additionally on membrane properties. Our work sheds light on how COPII transports the diverse range of cargo molecules required by the cell.Competing Interest StatementThe authors have declared no competing interest.