PT - JOURNAL ARTICLE AU - Carlo N. G. Giachello AU - Aref Arzan Zarin AU - Hiroshi Kohsaka AU - Yuen Ngan Fan AU - Akinao Nose AU - Matthias Landgraf AU - Richard A. Baines TI - Electrophysiological validation of premotor interneurons monosynaptically connected to the aCC motoneuron in the <em>Drosophila</em> larval CNS AID - 10.1101/2020.06.17.156430 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.06.17.156430 4099 - http://biorxiv.org/content/early/2020/06/19/2020.06.17.156430.short 4100 - http://biorxiv.org/content/early/2020/06/19/2020.06.17.156430.full AB - Mapping the wired connectivity of a nervous system is a prerequisite for full understanding of function. In this respect, such endeavours can be likened to genome sequencing projects. These projects similarly produce impressive amounts of data which, whilst a technical tour-de-force, remain under-utilised without validation. Validation of neuron synaptic connectivity requires electrophysiology which has the necessary temporal and spatial resolution to map synaptic connectivity. However, this technique is not common and requires extensive equipment and training to master, particularly when applied to the small CNS of the Drosophila larva. Thus, validation of connectivity in this CNS has been more reliant on behavioural analyses and, in particular, activity imaging using the calcium-sensor GCaMP. Whilst both techniques are powerful, they each have significant limitations for this purpose. Here we use electrophysiology to validate an array of driver lines reported to label specific premotor interneurons that the Drosophila connectome project suggests are monosynaptically connected to an identified motoneuron termed the anterior corner cell (aCC). Our results validate this proposition for four selected lines. Thus, in addition to validating the connectome with respect to these four premotor interneurons, our study highlights the need to functionally validate driver lines prior to use.Competing Interest StatementThe authors have declared no competing interest.