RT Journal Article
SR Electronic
T1 Genetic and chemical differentiation of Campylobacter coli and Campylobacter jejuni lipooligosaccharide pathways
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 050328
DO 10.1101/050328
A1 Alejandra Culebro
A1 Joana Revez
A1 Ben Pascoe
A1 Yasmin Friedmann
A1 Matthew D Hitchings
A1 Jacek Stupak
A1 Samuel K Sheppard
A1 Jianjun Li
A1 Mirko Rossi
YR 2016
UL http://biorxiv.org/content/early/2016/04/26/050328.abstract
AB Despite the importance of lipooligosaccharides (LOS) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in C. coli. In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species. Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to identify mechanisms consistent with the generation of LOS phenotypic heterogeneity. After classifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought. This genetic diversity is at the basis of a completely unexplored LOS structure heterogeneity. Mass spectrometry analysis of the LOS of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from C. jejuni’s. GlcN-GlcN disaccharides were present in the lipid A backbone in contrast to the GlcN3N-GlcN backbone observed in C. jejuni. Moreover, despite that many of the genes putatively involved in Qui3pNAcyl were absence in the genomes of various isolates, this rare sugar was found in the outer core of all C. coli. Therefore, regardless the high genetic diversity of LOS biosynthes is locus in C. coli, we identified species-specific phenotypic features of C. coli LOS which might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation.Depositories (where applicable) The whole genome sequences of C. coli are publicly available on the RAST server (http://rast.nmpdr.org) with guest account (login and password ‘guest’) under IDs: 195.91, 195.96-195.119, 195.124-195.126, 195.128-195.130, 195.133, 195.134, 6666666.94320