TY - JOUR T1 - Global lysine acetylation in <em>Escherichia coli</em> results from growth conditions that favor acetate fermentation JF - bioRxiv DO - 10.1101/457929 SP - 457929 AU - Birgit Schilling AU - Nathan Basisty AU - David G. Christensen AU - Dylan Sorensen AU - James S. Orr AU - Alan J. Wolfe AU - Christopher V. Rao Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/10/31/457929.abstract N2 - Lysine acetylation is thought to provide a mechanism for regulating metabolism in diverse bacteria. Indeed, many studies have shown that the majority of enzymes involved in central metabolism are acetylated and that acetylation can alter enzyme activity. However, the details regarding this regulatory mechanism are still unclear, specifically with regards to the signals that induce lysine acetylation. To better understand this global regulatory mechanism, we profiled changes in lysine acetylation during growth of Escherichia coli on the hexose glucose or the pentose xylose at both high and low sugar concentrations using label-free mass spectrometry. The goal was to see whether lysine acetylation differed during growth on these two different sugars. No significant differences, however, were observed. Rather, the initial sugar concentration was the principal factor governing changes in lysine acetylation, with higher sugar concentrations causing more acetylation. These results suggest that acetylation does not target specific metabolic pathways but rather simply targets accessible lysines, which may or may not alter enzyme activity. They further suggest that lysine acetylation principally results from conditions that favor accumulation of acetyl phosphate, the principal acetate donor in E. coli.IMPORTANCE Bacteria alter their metabolism in response to nutrient availability, growth conditions, and environmental stresses. This process is best understood at the level of transcriptional regulation, where many metabolic genes are conditionally expressed in response to diverse cues. However, additional modes of regulations are known to exist. One is lysine acetylation, a post-translational modification known to target many metabolic enzymes. However, unlike transcriptional regulation, little is known about this regulatory mode. We investigated the factors inducing changes in lysine acetylation by comparing growth on glucose and xylose. We found that the specific sugar used for growth did not alter the pattern of acetylation; rather, the principal factor was the amount of sugar, with more sugar yielding more acetylation. These results imply lysine acetylation is a global regulatory mechanism that is not responsive to the specific carbon source per se but rather the accumulation of downstream metabolites. ER -