RT Journal Article
SR Electronic
T1 Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 201731
DO 10.1101/201731
A1 Panfilio, Kristen A.
A1 Jentzsch, Iris M. Vargas
A1 Benoit, Joshua B.
A1 Erezyilmaz, Deniz
A1 Suzuki, Yuichiro
A1 Colella, Stefano
A1 Robertson, Hugh M.
A1 Poelchau, Monica F.
A1 Waterhouse, Robert M.
A1 Ioannidis, Panagiotis
A1 Weirauch, Matthew T.
A1 Hughes, Daniel S.T.
A1 Murali, Shwetha C.
A1 Werren, John H.
A1 Jacobs, Chris G.C.
A1 Duncan, Elizabeth J.
A1 Armisén, David
A1 Vreede, Barbara M.I.
A1 Baa-Puyoulet, Patrice
A1 Berger, Chloé S.
A1 Chang, Chun-che
A1 Chao, Hsu
A1 Chen, Mei-Ju M.
A1 Chen, Yen-Ta
A1 Childers, Christopher P.
A1 Chipman, Ariel D.
A1 Cridge, Andrew G.
A1 Crumière, Antonin J.J.
A1 Dearden, Peter K.
A1 Didion, Elise M.
A1 Dinh, Huyen
A1 Doddapaneni, HarshaVardhan
A1 Dolan, Amanda
A1 Dugan, Shannon
A1 Extavour, Cassandra G.
A1 Febvay, Gérard
A1 Friedrich, Markus
A1 Ginzburg, Neta
A1 Han, Yi
A1 Heger, Peter
A1 Holmes, Christopher J.
A1 Horn, Thorsten
A1 Hsiao, Yi-min
A1 Jennings, Emily C.
A1 Johnston, J. Spencer
A1 Jones, Tamsin E.
A1 Jones, Jeffery W.
A1 Khila, Abderrahman
A1 Koelzer, Stefan
A1 Kovacova, Viera
A1 Leask, Megan
A1 Lee, Sandra L.
A1 Lee, Chien-Yueh
A1 Lovegrove, Mackenzie R.
A1 Lu, Hsiao-ling
A1 Lu, Yong
A1 Moore, Patricia J.
A1 Munoz-Torres, Monica C.
A1 Muzny, Donna M.
A1 Palli, Subba R.
A1 Parisot, Nicolas
A1 Pick, Leslie
A1 Porter, Megan
A1 Qu, Jiaxin
A1 Refki, Peter N.
A1 Richter, Rose
A1 Pomar, Rolando Rivera
A1 Rosendale, Andrew J.
A1 Roth, Siegfried
A1 Sachs, Lena
A1 Santos, M. Emília
A1 Seibert, Jan
A1 Sghaier, Essia
A1 Shukla, Jayendra N.
A1 Stancliffe, Richard J.
A1 Tidswell, Olivia
A1 Traverso, Lucila
A1 Zee, Maurijn van der
A1 Viala, Séverine
A1 Worley, Kim C.
A1 Zdobnov, Evgeny M.
A1 Gibbs, Richard A.
A1 Richards, Stephen
YR 2018
UL http://biorxiv.org/content/early/2018/10/31/201731.abstract
AB Background The Hemiptera (aphids, cicadas, and true bugs) are a key insect order, with high diversity for feeding ecology and excellent experimental tractability for molecular genetics. Building upon recent sequencing of hemipteran pests such as phloem-feeding aphids and blood-feeding bed bugs, we present the genome sequence and comparative analyses centered on the milkweed bug Oncopeltus fasciatus, a seed feeder of the family Lygaeidae.Results The 926-Mb Oncopeltus genome is well represented by the current assembly and official gene set. We use our genomic and RNA-seq data not only to characterize the protein-coding gene repertoire and perform isoform-specific RNAi, but also to elucidate patterns of molecular evolution and physiology. We find ongoing, lineage-specific expansion and diversification of repressive C2H2 zinc finger proteins. The discovery of intron gain and turnover specific to the Hemiptera also prompted evaluation of lineage and genome size as predictors of gene structure evolution. Furthermore, we identify enzymatic gains and losses that correlate with feeding biology, particularly for reductions associated with derived, fluid-nutrition feeding.Conclusions With the milkweed bug, we now have a critical mass of sequenced species for a hemimetabolous insect order and close outgroup to the Holometabola, substantially improving the diversity of insect genomics. We thereby define commonalities among the Hemiptera and delve into how hemipteran genomes reflect distinct feeding ecologies. Given Oncopeltus's strength as an experimental model, these new sequence resources bolster the foundation for molecular research and highlight technical considerations for the analysis of medium-sized invertebrate genomes.