RT Journal Article SR Electronic T1 TLR3 deficiency exacerbates the loss of epithelial barrier function during genital tract Chlamydia muridarum infection JF bioRxiv FD Cold Spring Harbor Laboratory SP 459636 DO 10.1101/459636 A1 Ramesh Kumar A1 Haoli Gong A1 Luyao Liu A1 Nicole Ramos-Solis A1 Cheikh I. Seye A1 Wilbert A. Derbigny YR 2018 UL http://biorxiv.org/content/early/2018/11/01/459636.abstract AB Problem Chlamydia trachomatis infections are often associated with acute syndromes including cervicitis, urethritis, and endometritis, which can lead to chronic sequalae such as pelvic inflammatory disease (PID), chronic pelvic pain, ectopic pregnancy, and tubal infertility. As epithelial cells are the major cell type productively infected during genital tract Chlamydia infections, we investigated whether Chlamydia has any impact on the integrity of the host epithelial barrier as a possible mechanism to facilitate the dissemination of infection, and examined whether TLR3 function modulates its impact.Method of Study We used wild-type and TLR3-deficient murine oviduct epithelial (OE) cells to ascertain whether C. muridarum infection had any effect on the epithelial barrier integrity of these cells as measured by transepithelial resistance (TER) and cell permeability assays. We next assessed whether infection impacted the transcription and protein function of the cellular tight-junction (TJ) genes for claudins1-4, ZO-1, JAM1 and occludin via quantitative real-time PCR (qPCR) and western blot.Results qPCR, immunoblotting, transwell permeability assays, and TER studies show that Chlamydia compromises cellular TJ function throughout the course of infection in murine OE cells, and that TLR3 deficiency significantly exacerbates this effect.Conclusion Our data show that TLR3 plays a role in modulating epithelial barrier function during Chlamydia infection of epithelial cells lining the genital tract. This proposes a role for TLR3 signaling in maintaining the integrity of epithelial barrier function during genital tract Chlamydia infection, a function that we hypothesize is important in helping limit the chlamydial spread and subsequent genital tract pathology.