RT Journal Article
SR Electronic
T1 Single cell sequencing of the small and AT-skewed genome of malaria parasites
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.02.21.960039
DO 10.1101/2020.02.21.960039
A1 Shiwei Liu
A1 Adam C. Huckaby
A1 Audrey C. Brown
A1 Christopher C. Moore
A1 Ian Burbulis
A1 Michael J. McConnell
A1 Jennifer L. Güler
YR 2020
UL http://biorxiv.org/content/early/2020/06/21/2020.02.21.960039.abstract
AB Single cell genomics is a rapidly advancing field; however, most techniques are designed for mammalian cells. Here, we present a single cell sequencing pipeline for the intracellular parasite, Plasmodium falciparum, which harbors a relatively small genome with an extremely skewed base content. Through optimization of a quasi-linear genome amplification method, we achieve better targeting of the parasite genome over contaminants and generate coverage levels that allow detection of relatively small copy number variations on a single cell level. These improvements are important for expanding accessibility of single cell approaches to new organisms and for improving the study of adaptive mechanisms.Competing Interest StatementThe authors have declared no competing interest.