RT Journal Article
SR Electronic
T1 Efficient reverse breeding by VIGS-mediated transient crossover reduction
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 459016
DO 10.1101/459016
A1 Calvo-Baltanas, Vanesa
A1 Wijnen, Cris L.
A1 Lukhovitskaya, Nina
A1 de Snoo, C. Bastiaan
A1 Hohenwarter, Linus
A1 de jong, Hans
A1 Schnittger, Arp
A1 Wijnker, Erik
YR 2018
UL http://biorxiv.org/content/early/2018/11/01/459016.abstract
AB F1 heterozygotes are traditionally generated by crossing homozygous parental lines. The opposite is achieved through reverse breeding, in which parental lines are generated from a heterozygote. Reverse breeding can be used to develop new F1 hybrid varieties without having prior access to homozygous breeding lines. For successful reverse breeding, the heterozygotes’ homologous chromosomes must be divided over two haploid complements, which is achieved by suppression of meiotic crossover (CO) recombination. We here show two innovations that facilitate efficient reverse breeding. Firstly, we demonstrate that downregulation of CO rates can be accomplished using virus-induced gene silencing (VIGS). We obtain transgene-free parental lines for a heterozygote in just two generations. Secondly, we show that incomplete CO suppression opens up several alternative strategies for the preservation of hybrid phenotypes through reverse breeding.