TY - JOUR T1 - Keratins couple with the nuclear lamina and regulate proliferation in colonic epithelial cells JF - bioRxiv DO - 10.1101/2020.06.22.164467 SP - 2020.06.22.164467 AU - Carl-Gustaf A. Stenvall AU - Joel H. Nyström AU - Ciarán Butler-Hallissey AU - Stephen A. Adam AU - Roland Foisner AU - Karen M. Ridge AU - Robert D. Goldman AU - Diana M. Toivola Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/06/22/2020.06.22.164467.abstract N2 - Keratin intermediate filaments (IFs) convey mechanical stability and protection against stress to epithelial cells, and may participate in nuclear structure and organization. Keratins are important for colon health as observed in keratin 8 knockout (K8−/−) mice, which exhibit colonic inflammation and epithelial hyperproliferation. Here, using a full body and two intestinal epithelial-specific K8−/− knockout mouse models, we determine if cytoplasmic keratins affect the nuclear structure and lamina in epithelial colonocytes. K8−/− colonocytes in vivo and in organoid cultures exhibit significantly decreased levels of the major lamins A/C, B1 and B2 in a colon-specific and cell-intrinsic manner independent of major changes in colonic inflammation or microbiota. Downregulation of K8 by siRNA in Caco-2 cells similarly decreases lamin A levels, which recover after re-expression of K8. K8 loss is associated with reduced plectin, LINC complex proteins and lamin-associated proteins, indicating a dysfunctional keratin-nuclear lamina coupling. Immunoprecipitation identifies complexes of colonocyte keratins with the LINC protein SUN2 and lamin A. Hyperphosphorylation of the lamin A-associated cell cycle regulator pRb in K8−/− colonocytes together with increased nuclear localization of the mechanosensor YAP provide a molecular mechanism for the hyperproliferation phenotype. These findings identify a novel, colonocyte-specific role for K8 in nuclear function.Competing Interest StatementThe authors have declared no competing interest. ER -