RT Journal Article
SR Electronic
T1 The LIM-Only Protein FHL2 is Involved in Autophagy to Regulate the Development of Skeletal Muscle
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 459800
DO 10.1101/459800
A1 Zihao Liu
A1 Shunshun Han
A1 Yan Wang
A1 Haorong He
A1 Xiaoxu Shen
A1 Yuqi Chen
A1 Can Cui
A1 Yao Zhang
A1 Lin Ye
A1 Diyan Li
A1 Xiaoling Zhao
A1 Huarui Du
A1 Xiaosong Jiang
A1 Chunlin Yu
A1 Qingyun Li
A1 Qing Zhu
A1 Chaowu Yang
A1 Huadong Yin
YR 2018
UL http://biorxiv.org/content/early/2018/11/02/459800.abstract
AB Four and a half LIM domain protein 2 (FHL2) is a LIM domain protein expressed in muscle tissue whose deletion is causative of myopathies. Although FHL2 has a confirmed important role in muscle development, its autophagy-related function in muscle differentiation has not been fully determined. To explore the role of FHL2 in autophagy-related muscle regulation, FHL2-silenced and -overexpressing C2C12 mouse cells were examined. Immunofluorescence and coimmunoprecipitation assay findings showed that FHL2 silencing reduced LC3-II protein expression and the amount of LC3 that coimmunoprecipitated with FHL2, indicating that FHL2 interacts with LC3-II in the formation of autophagosomes. Moreover, the expression of muscle development marker genes such as MyoD1 and MyoG was lower in FHL2-silenced C2C12 cells but not in FHL2-overexpressing C2C12 cells. Electron microscopy analysis revealed large empty autophagosomes in FHL2-silenced myoblasts, while flow cytometry suggested that FHL2 silencing made cells more vulnerable to staurosporine-induced cell death. In conclusion, we propose that FHL2 interacts with LC3-II in autophagosome formation to regulate the development of muscle cells.