PT  - JOURNAL ARTICLE
AU  - Martignano, Filippo
AU  - Crucitta, Stefania
AU  - Mingrino, Alessandra
AU  - Semeraro, Roberto
AU  - Del Re, Marzia
AU  - Petrini, Iacopo
AU  - Magi, Alberto
AU  - Conticello, Silvestro G.
TI  - Nanopore sequencing from liquid biopsy: analysis of copy number variations from cell-free DNA of lung cancer patients
AID  - 10.1101/2020.06.22.165555
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.06.22.165555
4099  - http://biorxiv.org/content/early/2020/06/23/2020.06.22.165555.short
4100  - http://biorxiv.org/content/early/2020/06/23/2020.06.22.165555.full
AB  - Alterations in the genetic content, such as Copy Number Variations (CNVs) is one of the hallmarks of cancer and their detection is used to recognize tumoral DNA. Analysis of cell-free DNA from plasma is a powerful tool for non-invasive disease monitoring in cancer patients. Here we exploit third generation sequencing (Nanopore) to obtain a CNVs profile of tumoral DNA from plasma, where cancer-related chromosomal alterations are readily identifiable.Compared to Illumina sequencing -the only available alternative- Nanopore sequencing represents a viable approach to characterize the molecular phenotype, both for its ease of use, costs and rapid turnaround (6 hours).Competing Interest StatementThe authors have declared no competing interest.