TY - JOUR T1 - Resilience to oxidative and nitrosative stress is mediated by the stressosome, RsbP and SigB in <em>Bacillus subtilis.</em> JF - bioRxiv DO - 10.1101/460303 SP - 460303 AU - Vina Tran AU - Kara Geraci AU - Giovanni Midili AU - William Satterwhite AU - Rachel Wright AU - Carla Yaneth Bonilla Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/11/02/460303.abstract N2 - A bacterium’s ability to thrive in the presence of multiple environmental stressors simultaneously determines its resilience. We showed that activation of the SigB-controlled general stress response by mild environmental or nutritional stress provided significant cross-protection to subsequent lethal oxidative, disulfide and nitrosative stress exposure. SigB activation is mediated via the stressosome and RsbP, the main conduits of environmental and nutritional stress, respectively. Cells exposed to mild environmental stress while lacking the major stressosome components RsbT or RsbRA were highly sensitive to subsequent oxidative stress, whereas rsbRB, rsbRC, rsbRD and ytvA null mutants showed a spectrum of sensitivity, confirming their redundant roles and suggesting they could modulate the signal generated by environmental stress or oxidative stress. Furthermore, from mutant analysis we infer that RsbRA phosphorylation by RsbT was important for this cross-resistance to oxidative stress. By contrast, cells encountering stationary phase stress required RsbP but not RsbT to survive subsequent oxidative stress caused by hydrogen peroxide and diamide. Interestingly, optimum cross-protection against nitrosative stress caused by SNP required SigB but not the known regulators, RsbT and RsbP, suggesting an additional and as yet uncharacterized route of SigB activation independent of the known environmental and energy-stress pathways. Together, these results provide a mechanism for how Bacillus subtilis promotes enhanced resistance against lethal oxidative stress during likely physiologically relevant conditions such as mild environmental or nutrient stress. ER -