RT Journal Article
SR Electronic
T1 The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.06.23.167163
DO 10.1101/2020.06.23.167163
A1 Yin Ho Vong
A1 Lavanya Sivashanmugam
A1 Andreas Zaucker
A1 Alex Jones
A1 Karuna Sampath
YR 2020
UL http://biorxiv.org/content/early/2020/06/24/2020.06.23.167163.abstract
AB The ability to reproduce is essential in all branches of life. In metazoans, this process is initiated by formation of the germline, a group of cells that are destined to form the future gonads, the tissue that will produce the gametes. The molecular mechanisms underlying germline formation differs between species. In zebrafish, development of the germline is dependent on the specification, migration and proliferation of progenitors called the primordial germ cells (PGCs). PGC specification is dependent on a maternally provided cytoplasmic complex of ribonucleoproteins (RNPs), the germplasm. Here, we show that the conserved RNA-binding protein (RBP), Igf2bp3, has an essential role during early embryonic development and germline development. Loss of Igf2bp3 leads to an expanded yolk syncytial layer (YSL) in early embryos, reduced germline RNA expression, and mis-regulated germline development. Maternal mutants affecting igf2bp3 exhibit abnormal PGCs and adult igf2bp3 mutants show male biased sex ratios. Therefore, Igf2bp3 is required for normal embryonic and germline development.Competing Interest StatementThe authors have declared no competing interest.