TY - JOUR T1 - <em>In vitro</em> characterization of the human segmentation clock JF - bioRxiv DO - 10.1101/461822 SP - 461822 AU - Margarete Diaz-Cuadros AU - Daniel E Wagner AU - Christoph Budjan AU - Alexis Hubaud AU - Jonathan Touboul AU - Arthur Michaut AU - Ziad Al Tanoury AU - Kumiko Yoshioka-Kobayashi AU - Yusuke Niino AU - Ryoichiro Kageyama AU - Atsushi Miyawaki AU - Olivier Pourquié Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/11/04/461822.abstract N2 - The vertebral column is characterized by the periodic arrangement of vertebrae along the anterior-posterior (AP) axis. This segmental or metameric organization is established early in embryogenesis when pairs of embryonic segments called somites are rhythmically produced by the presomitic mesoderm (PSM). The tempo of somite formation is controlled by a molecular oscillator known as the segmentation clock 1,2. While this oscillator has been well characterized in model organisms 1,2, whether a similar oscillator exists in humans remains unknown. We have previously shown that human embryonic stem (ES) cells or induced pluripotent stem (iPS) cells can differentiate in vitro into PSM upon activation of the Wnt signaling pathway combined with BMP inhibition3. Here, we show that these human PSM cells exhibit Notch and YAP-dependent oscillations4 of the cyclic gene HES7 with a 5-hour period. Single cell RNA-sequencing comparison of the differentiating iPS cells with mouse PSM reveals that human PSM cells follow a similar differentiation path and exhibit a remarkably coordinated differentiation sequence. We also demonstrate that FGF signaling controls the phase and period of the oscillator. This contrasts with classical segmentation models such as the “Clock and Wavefront” 1,2,5, where FGF merely implements a signaling threshold specifying where oscillations stop. Overall, our work identifying the human segmentation clock represents an important breakthrough for human developmental biology. ER -