TY - JOUR T1 - Deciphering the regulatory logic of a <em>Drosophila</em> enhancer through systematic sequence mutagenesis and quantitative image analysis JF - bioRxiv DO - 10.1101/2020.06.24.169748 SP - 2020.06.24.169748 AU - Yann Le Poul AU - Yaqun Xin AU - Liucong Ling AU - Bettina Mühling AU - Rita Jaenichen AU - David Hörl AU - David Bunk AU - Hartmann Harz AU - Heinrich Leonhardt AU - Yingfei Wang AU - Elena Osipova AU - Mariam Museridze AU - Deepak Dharmadhikari AU - Eamonn Murphy AU - Remo Rohs AU - Stephan Preibisch AU - Benjamin Prud’homme AU - Nicolas Gompel Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/06/25/2020.06.24.169748.abstract N2 - Transcriptional enhancers are short DNA sequences controlling the spatial activity, timing and levels of eukaryotic gene transcription. Their quantitative transcriptional output is thought to result from the number and organization of transcription factor binding sites (TFBSs). Yet, how the various aspects of regulatory information are encoded in enhancer sequences remains elusive. We addressed this question by quantifying the spatial activity of the yellow spot enhancer active in developing Drosophila wings. To identify which enhancer DNA sequence contributes to enhancer activity, we introduced systematic mutations along the enhancer. We developed an analytic framework that uses comprehensive descriptors to quantify reporter assay in transgenic flies and measure spatial variations in activity levels across the wing. Our analysis highlights an unexpected density of regulatory information in the spot enhancer sequence. Furthermore, it reveals an unanticipated regulatory logic underlying the activity of this enhancer, and how it reads the wing trans-regulatory landscape to encode a spatial pattern.Competing Interest StatementThe authors have declared no competing interest. ER -