TY - JOUR T1 - Characterization of oral microbiota in marmosets: feasibility of using the marmoset as a human oral disease model JF - bioRxiv DO - 10.1101/462911 SP - 462911 AU - Sachiko Takehara AU - Jorge L Zeredo AU - Yasuhiro Kumei AU - Kensuke Kagiyama AU - Kazumasa Fukasawa AU - Akiko Oshiro AU - Masayuki Ueno AU - Shunsuke Minakuchi AU - Yoko Kawaguchi Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/11/05/462911.abstract N2 - As the world’s population is aging, there is an increasing demand for research regarding aging and aging-related disorders, to achieve better understanding of aging. Ideally, such research would be performed with human subjects. However, due to ethical considerations, animals such as rodents and monkeys are used as alternatives. Among these alternative models, non-human primates are preferred because they share common traits with humans. The small South American common marmoset (Callithrix jacchus) may offer a number of advantages over other non-human primates in terms of its relatively small size, short life span, and identical dental anatomy, compared with humans. The purpose of this study was to clarify the viability of using the marmoset as a human oral disease model.We collected saliva samples from eight common marmosets and eight human subjects. Prokaryotic DNA was extracted from the saliva samples, and 16S bacterial rRNA gene sequencing was performed on each of the samples. Our results indicated that the types of oral microbiomes detected among human and marmoset samples were nearly identical. In contrast, the oral microbiomes of our human and marmoset subjects were distinctly different from those of rat and dogs, which are popular animal models. The oral microbiomes of marmosets showed greater diversity than those of humans. With respect to individual variation, marmosets exhibited less variation in their oral flora, compared with humans. This difference in variation might be attributed to the fact that marmoset subjects were kept in a controlled environment with identical lifestyles.The characteristics of its oral microbiota, combined with other technical advantages, suggest that the marmoset may provide the best animal model thus far for the study of oral health. This study characterized the oral microbes of the marmoset, thereby providing information to support future application of the marmoset as a model for age-related oral disease. ER -