RT Journal Article
SR Electronic
T1 BiSCoT: Improving large eukaryotic genome assemblies with optical maps
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 674721
DO 10.1101/674721
A1 Benjamin Istace
A1 Caroline Belser
A1 Jean-Marc Aury
YR 2020
UL http://biorxiv.org/content/early/2020/07/01/674721.abstract
AB Motivation Long read sequencing and Bionano Genomics optical maps are two techniques that, when used together, make it possible to reconstruct entire chromosome or chromosome arms structure. However, the existing tools are often too conservative and organization of contigs into scaffolds is not always optimal.Results We developed BiSCoT (Bionano SCaffolding COrrection Tool), a tool that post-processes files generated during a Bionano scaffolding in order to produce an assembly of greater contiguity and quality. BiSCoT was tested on a human genome and four publicly available plant genomes sequenced with Nanopore long reads and improved significantly the contiguity and quality of the assemblies. BiSCoT generates a fasta file of the assembly as well as an AGP file which describes the new organization of the input assembly.Availability BiSCoT and improved assemblies are freely available on Github at http://www.genoscope.cns.fr/biscot and Pypi at https://pypi.org/project/biscot/.Competing Interest StatementThe authors have declared no competing interest.