RT Journal Article
SR Electronic
T1 RNA is a critical element for the sizing and the composition of phase-separated RNA-protein condensates
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 457986
DO 10.1101/457986
A1 Marina Garcia-Jove Navarro
A1 Shunnichi Kashida
A1 Racha Chouaib
A1 Sylvie Souquere
A1 Gerard Pierron
A1 Dominique Weil
A1 Zoher Gueroui
YR 2018
UL http://biorxiv.org/content/early/2018/11/08/457986.abstract
AB Liquid-liquid phase separation is thought to be a key organizing principle in eukaryotic cells to generate highly concentrated dynamic assemblies, such as the RNP granules. Numerous in vitro approaches have validated this model, yet a missing aspect is to take into consideration the complex molecular mixture and promiscuous interactions found in vivo. Here we report the versatile scaffold “ArtiG” to generate concentration-dependent RNA-protein condensates within living cells, as a bottom-up approach to study the impact of co-segregated endogenous components on phase separation. We demonstrate that intracellular RNA seeds the nucleation of the condensates, as it provides molecular cues to locally coordinate the formation of endogenous high order RNP assemblies. Interestingly, the co-segregation of intracellular components ultimately impacts the size of the phase-separated condensates. Thus, RNA arises as an architectural element that can influence the composition and the morphological outcome of the condensate phases in an intracellular context.