TY - JOUR T1 - Adult Canine Intestinal Derived Organoids: A Novel <em>In Vitro</em> System for Translational Research in Comparative Gastroenterology JF - bioRxiv DO - 10.1101/466409 SP - 466409 AU - Lawrance Chandra AU - Dana C Borcherding AU - Dawn Kingsbury AU - Todd Atherly AU - Yoko M Ambrosini AU - Agnes Bourgois-Mochel AU - Wang Yuan AU - Michael Kimber AU - Yijun Qi AU - Qun Wang AU - Michael Wannemuehler AU - N Matthew Ellinwood AU - Elizabeth Snella AU - Martin Martin AU - Melissa Skala AU - David Meyerholz AU - Mary Estes AU - Martin E. Fernandez-Zapico AU - Albert E. Jergens AU - Jonathan P Mochel AU - Karin Allenspach Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/11/09/466409.abstract N2 - Background Large animal models, such as the dog, are increasingly being used over rodent models for studying naturally occurring diseases including gastrointestinal (GI) disorders. Dogs share similar environmental, genomic, anatomical, and intestinal physiologic features with humans. To bridge the gap between currently used animal models (e.g. mouse) and humans, and expand the translational potential of the dog model, we developed a three dimensional (3D) canine GI organoid (enteroid and colonoid) system. Organoids have recently gained interest in translational research as this model system better recapitulates the physiological and molecular features of the tissue environment in comparison with two-dimensional cultures.Results Organoids were propagated from isolation of adult intestinal stem cells (ISC) from whole jejunal tissue as well as endoscopically obtained duodenal, ileal and colonic biopsy samples of healthy dogs and GI cases, including inflammatory bowel disease (IBD) and intestinal carcinomas. Intestinal organoids were comprehensively characterized using histology, immunohistochemistry, RNA in situ hybridization and transmission electron microscopy, and organoids mimicked the in vivo tissue environment. Physiological relevance of the enteroid system was defined using functional assays such as Optical Metabolic Imaging (OMI), the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) function assay, and Exosome-Like Vesicles (EV) uptake assay, as a basis for wider applications of this technology in basic, preclinical and translational GI research.Conclusions In summary, our findings establish the canine GI organoid systems as a novel model to study naturally occurring intestinal diseases in dogs and humans. Furthermore, canine organoid systems will help to elucidate host-pathogen interactions contributing to GI disease pathogenesis. ER -