PT  - JOURNAL ARTICLE
AU  - Alexis M. Ceasrine
AU  - Eugene E. Lin
AU  - David N. Lumelsky
AU  - Nelmari Ruiz-Otero
AU  - Erica D. Boehm
AU  - Rejji Kuruvilla
TI  - Tamoxifen improves glucose tolerance in a delivery, sex, and strain-dependent manner in mice
AID  - 10.1101/467266
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 467266
4099  - http://biorxiv.org/content/early/2018/11/09/467266.short
4100  - http://biorxiv.org/content/early/2018/11/09/467266.full
AB  - Tamoxifen, a selective estrogen receptor modulator, is widely used in mouse models to temporally control gene expression but is also known to affect body composition. Here, we report that tamoxifen has significant and sustained effects on glucose tolerance, independent of effects on insulin sensitivity, in mice. Intraperitoneal, but not oral, tamoxifen delivery improved glucose tolerance in three inbred mouse strains. The extent and persistence of tamoxifen-induced effects were sex- and strain-dependent. These findings highlight the need to revise commonly used tamoxifen-based protocols for gene manipulation in mice by including longer chase periods following injection, oral delivery, and the use of tamoxifen-treated littermate controls.