PT  - JOURNAL ARTICLE
AU  - Sarah Davidson
AU  - Mirjana Efremova
AU  - Angela Riedel
AU  - Bidesh Mahata
AU  - Jhuma Pramanik
AU  - Jani Huuhtanen
AU  - Gozde Kar
AU  - Roser Vento-Tormo
AU  - Tzachi Hagai
AU  - Xi Chen
AU  - Muzlifah A. Haniffa
AU  - Jacqueline D. Shields
AU  - Sarah A. Teichmann
TI  - Single-cell RNA sequencing reveals a dynamic stromal niche within the evolving tumour microenvironment
AID  - 10.1101/467225
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 467225
4099  - http://biorxiv.org/content/early/2018/11/09/467225.short
4100  - http://biorxiv.org/content/early/2018/11/09/467225.full
AB  - Non-cancerous stromal cells represent a highly diverse compartment of the tumour, yet their role across tumour evolution remains unclear. We employed single-cell RNA sequencing to determine stromal adaptations in murine melanoma at different points of tumour development. Naive lymphocytes recruited from lymph nodes underwent activation and clonal expansion within the tumour, prior to PD1 and Lag3 expression, while tumour-associated myeloid cells promoted the formation of a suppressive niche through cytokine secretion and inhibitory T cell interactions. We identified three temporally distinct cancer-associated fibroblast (CAF) populations displaying unique signatures, and verified these in human datasets. In early tumours, immune CXCL12/CSF1 and complement-expressing CAFs supported recruitment of macrophages, whereas contractile CAFs became more prevalent in later tumours. This study highlights the complex interplay and increasing diversity among cells that co-evolve with the tumour, indicating that from early stages of development, stromal cells acquire the capacity to modulate the immune landscape towards suppression.