TY - JOUR T1 - The LIM-Only Protein FHL2 is involved in autophagy to regulate the development of skeletal muscle cell JF - bioRxiv DO - 10.1101/459800 SP - 459800 AU - Zihao Liu AU - Shunshun Han AU - Yan Wang AU - Can Cui AU - Qing Zhu AU - Xiaosong Jiang AU - Chaowu Yang AU - Huarui Du AU - Chunlin Yu AU - Qingyun Li AU - Haorong He AU - Xiaoxu Shen AU - Yuqi Chen AU - Yao Zhang AU - Lin Ye AU - Zhichao Zhang AU - Diyan Li AU - Xiaoling Zhao AU - Huadong Yin Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/11/09/459800.abstract N2 - Four and a half LIM domain protein 2 (FHL2) is a LIM domain protein expressed in muscle tissue whose deletion is causative of myopathies. Although FHL2 has a confirmed important role in muscle development, its autophagy-related function in muscle differentiation has not been fully determined. To explore the role of FHL2 in autophagy-related muscle regulation, FHL2-silenced and -overexpressing C2C12 mouse cells were examined. Immunofluorescence and co-immunoprecipitation assay findings showed that FHL2 silencing reduced LC3-Ⅱ protein expression and the amount of LC3 that co-immunoprecipitated with FHL2, indicating that FHL2 interacts with LC3-Ⅱ in the formation of autophagosomes. Moreover, the expression of muscle development marker genes such as MyoD1 and MyoG was lower in FHL2-silenced C2C12 cells but not in FHL2-overexpressing C2C12 cells. Electron microscopy analysis revealed large empty autophagosomes in FHL2-silenced myoblasts, while flow cytometry suggested that FHL2 silencing made cells more vulnerable to staurosporine-induced cell death. In conclusion, we propose that FHL2 interacts with LC3-Ⅱ in autophagosome formation to regulate the development of muscle cells. ER -