%0 Journal Article %A Jianbo Zhang %A Yu-Ja Huang %A Jun-Young Yoon %A John Kemmitt %A Charles Wright %A Kirsten Schneider %A Pierre Sphabmixay %A Victor Hernandez-Gordillo %A Steven J. Holcomb %A Brij Bhushan %A Gar Rohatgi %A Kyle Benton %A David Carpenter %A Jemila C. Kester %A George Eng %A David T. Breault %A Omer Yilmaz %A Mao Taketani %A Christopher A. Voigt %A Rebecca L. Carrier %A David L. Trumper %A Linda G. Griffith %T Primary human colonic mucosal barrier crosstalk with super oxygen-sensitive Faecalibacterium prausnitzii in continuous culture %D 2020 %R 10.1101/2020.07.02.185082 %J bioRxiv %P 2020.07.02.185082 %X The gut microbiome plays an important role in human health and disease. Gnotobiotic animal and in vitro cell-based models provide some informative insights into mechanistic crosstalk. However, there is no existing system for a chronic co-culture of a human colonic mucosal barrier with super oxygen-sensitive commensal microbes, hindering the study of human-microbe interactions in a controlled manner. Here, we investigated the effects of an abundant super oxygen-sensitive commensal anaerobe, Faecalibacterium prausnitzii, on a primary human mucosal barrier using a Gut-MIcrobiome (GuMI) physiome platform that we designed and fabricated. Chronic continuous co-culture of F. prausnitzii for two days with colon epithelia, enabled by continuous flow of completely anoxic apical media and aerobic basal media, resulted in a strictly anaerobic apical environment fostering growth of and butyrate production by F. prausnitzii, while maintaining a stable colon epithelial barrier. We identified elevated differentiation and hypoxia-responsive genes and pathways in the platform compared with conventional aerobic static culture of the colon epithelia, attributable to a combination of anaerobic environment and continuous medium replenishment. Furthermore, we demonstrated anti-inflammatory effects of F. prausnitzii through HDAC and the TLR-NFKB axis. Finally, we identified that butyrate largely contributes to the anti-inflammatory effects by downregulating TLR3 and TLR4. Our results are consistent with some clinical observations regarding F. prausnitzii, thus motivating further studies employing this platform with more complex engineered colon tissues for understanding the interaction between the human colonic mucosal barrier and microbiota, pathogens, or engineered bacteria.Competing Interest StatementA provisional patent was filed. %U https://www.biorxiv.org/content/biorxiv/early/2020/07/03/2020.07.02.185082.full.pdf