TY - JOUR T1 - Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells JF - bioRxiv DO - 10.1101/328328 SP - 328328 AU - Mridusmita Saikia AU - Philip Burnham AU - Sara H. Keshavjee AU - Michael F. Z. Wang AU - Michael Heyang AU - Pablo Moral-Lopez AU - Meleana M. Hinchman AU - Charles G. Danko AU - John S. L. Parker AU - Iwijn De Vlaminck Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/11/13/328328.abstract N2 - We describe Droplet Assisted RNA Targeting by single cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes. ER -