PT  - JOURNAL ARTICLE
AU  - Kwan-Wood Gabriel Lam
AU  - Kevin Brick
AU  - Gang Cheng
AU  - Florencia Pratto
AU  - R. Daniel Camerini-Otero
TI  - Cell-type-specific genomics reveals histone modification dynamics in mammalian meiosis
AID  - 10.1101/469908
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 469908
4099  - http://biorxiv.org/content/early/2018/11/14/469908.short
4100  - http://biorxiv.org/content/early/2018/11/14/469908.full
AB  - Meiosis is the specialized cell division during which parental genomes recombine to create genotypically unique gametes. Despite its importance, mammalian meiosis cannot be studied in vitro, greatly limiting mechanistic studies. In vivo, meiocytes progress asynchronously through meiosis and therefore the study of specific stages of meiosis is a challenge. Here, we describe a simple method for isolating pure sub-populations of meiocytes that allows for detailed study of meiotic sub-stages. Interrogating the H3K4me3 landscape revealed dynamic chromatin transitions between sub-stages of meiotic prophase I, both at sites of genetic recombination and at gene promoters. We also leveraged this method to perform the first comprehensive, genome-wide survey of histone marks in meiotic prophase, revealing a heretofore unappreciated complexity of the epigenetic landscape at meiotic recombination hotspots. Ultimately, this study presents a simple, scalable framework for interrogating the complexities of mammalian meiosis.