RT Journal Article
SR Electronic
T1 B cells and HSV-specific antibodies respond to HSV-2 reactivation in skin
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.07.08.192542
DO 10.1101/2020.07.08.192542
A1 Emily S. Ford
A1 Anton M. Sholukh
A1 RuthMabel Boytz
A1 Savanna S. Carmack
A1 Alexis Klock
A1 Khamsone Phasouk
A1 Jason Shao
A1 Raabya Rossenkhan
A1 Paul T. Edlefsen
A1 Tao Peng
A1 Christine Johnston
A1 Anna Wald
A1 Jia Zhu
A1 Lawrence Corey
YR 2020
UL http://biorxiv.org/content/early/2020/07/08/2020.07.08.192542.abstract
AB Tissue-based T cells increasingly have been shown to be important effectors in the control and prevention of mucosal viral infections – less is known about tissue-based B cells. We demonstrate that B cells and antibody-secreting cells (ASCs) are present in skin biopsies of persons with symptomatic HSV-2 reactivation. CD20+ B cells are observed in inflammatory infiltrates at greatest density at the time of symptomatic reactivation; HSV-2-specific antibodies to HSV-2 surface antigens are also detected. The concentrations of HSV-2-specific antibodies in tissue biopsies vary over the course of HSV-2 reactivation and healing, unlike serum where concentrations remain static over time. B cells and HSV-specific antibody were rarely present in biopsies of unaffected skin. Investigation of serial biopsies over the course of lesion healing suggests that B cells follow a more migratory than resident pattern of infiltration in HSV-affected genital skin, in contrast to T cells. Together, these observations may suggest a functional and distinct role of tissue-based B cells in the local immune response to HSV-2.Competing Interest StatementThe authors have declared no competing interest.