RT Journal Article
SR Electronic
T1 The secreted protein signature of hydatid fluid from pulmonary cystic echinococcosis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.07.09.195701
DO 10.1101/2020.07.09.195701
A1 Guilherme Brzoskowski dos Santos
A1 Edileuza Danieli da Silva
A1 Eduardo Shigueo Kitano
A1 Maria Eduarda Battistella
A1 Karina Mariante Monteiro
A1 Jeferson Camargo de Lima
A1 Henrique Bunselmeyer Ferreira
A1 Solange Maria de Toledo Serrano
A1 Arnaldo Zaha
YR 2020
UL http://biorxiv.org/content/early/2020/07/10/2020.07.09.195701.abstract
AB The vast majority of cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Comparative proteomic studies of helminths have increased the knowledge about the molecular survival strategies adopted by parasites. Here, we surveyed the protein contents of the hydatid fluid compartment of E. granulosus and E. ortleppi pulmonary bovine cysts, in an attempt to compare their molecular arsenal in this host-parasite interface. Hydatid fluid samples from three isolates of each species were analyzed by trypsin digestion and mass spectrometry. We identified 280 proteins in E. granulosus and 251 proteins in E. ortleppi, highlighting a core of 52 proteins common to all samples of hydatid fluid. The in silico functional analysis revealed important molecular functions and processes active in pulmonary cystic echinococcosis. Some were more evident in one species, such as apoptosis in E. ortleppi, and cysteine protease activity in E. granulosus, while many molecular activities have been found in fluids of both species, such as proteolysis, development signaling and extracellular structures organization. The similar molecular tools employed by E. granulosus and E. ortleppi for their survival within the host are potential targets for new therapeutic approaches to deal with cystic echinococcosis and other larval cestodiases.Competing Interest StatementThe authors have declared no competing interest.