PT  - JOURNAL ARTICLE
AU  - Silas Maniatis
AU  - Tarmo Äijö
AU  - Sanja Vickovic
AU  - Catherine Braine
AU  - Kristy Kang
AU  - Annelie Mollbrink
AU  - Delphine Fagegaltier
AU  - Žaneta Andrusivová
AU  - Sami Saarenpää
AU  - Gonzalo Saiz-Castro
AU  - Miguel Cuevas
AU  - Aaron Watters
AU  - Joakim Lundeberg
AU  - Richard Bonneau
AU  - Hemali Phatnani
TI  - Spatiotemporal Dynamics of Molecular Pathology in Amyotrophic Lateral Sclerosis
AID  - 10.1101/389270
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 389270
4099  - http://biorxiv.org/content/early/2018/11/16/389270.short
4100  - http://biorxiv.org/content/early/2018/11/16/389270.full
AB  - Paralysis occurring in amyotrophic lateral sclerosis (ALS) results from denervation of skeletal muscle as a consequence of motor neuron degeneration. Interactions between motor neurons and glia contribute to motor neuron loss, but the spatiotemporal ordering of molecular events that drive these processes in intact spinal tissue remains poorly understood. Here, we use spatial transcriptomics to obtain gene expression measurements of mouse spinal cords over the course of disease, as well as of postmortem tissue from ALS patients, to characterize the underlying molecular mechanisms in ALS. We identify novel pathway dynamics, regional differences between microglia and astrocyte populations at early time-points, and discern perturbations in several transcriptional pathways shared between murine models of ALS and human postmortem spinal cords.One Sentence Summary Analysis of the ALS spinal cord using Spatial Transcriptomics reveals spatiotemporal dynamics of disease driven gene regulation.