PT  - JOURNAL ARTICLE
AU  - Romain F. Laine
AU  - Tessa Sinnige
AU  - Kai Yu Ma
AU  - Amanda J. Haack
AU  - Chetan Poudel
AU  - Peter Gaida
AU  - Nathan Curry
AU  - Michele Perni
AU  - Ellen A.A. Nollen
AU  - Christopher M. Dobson
AU  - Michele Vendruscolo
AU  - Gabriele S. Kaminski Schierle
AU  - Clemens F. Kaminski
TI  - Fast fluorescence lifetime imaging reveals the aggregation processes of α-synuclein and polyglutamine in aging <em>Caenorhabditis elegans</em>
AID  - 10.1101/414714
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 414714
4099  - http://biorxiv.org/content/early/2018/11/16/414714.short
4100  - http://biorxiv.org/content/early/2018/11/16/414714.full
AB  - The nematode worm Caenorhabditis elegans has emerged as an important model organism to study the molecular mechanisms of protein misfolding diseases associated with amyloid formation because of its small size, ease of genetic manipulation and optical transparency. Obtaining a reliable and quantitative read-out of protein aggregation in this system, however, remains a challenge. To address this problem, we here present a fast time-gated fluorescence lifetime imaging (TG-FLIM) method and show that it provides functional insights into the process of protein aggregation in living animals by enabling the rapid characterisation of different types of aggregates. More specifically, in longitudinal studies of C. elegans models of Parkinson’s and Huntington’s diseases, we observed marked differences in the aggregation kinetics and the nature of the protein inclusions formed by α-synuclein and polyglutamine. In particular, we found that α-synuclein inclusions do not display amyloid-like features until late in the life of the worms, whereas polyglutamine forms amyloid characteristics rapidly in early adulthood. Furthermore, we show that the TG-FLIM method is capable of imaging live and non-anaesthetised worms moving in specially designed agarose micro-chambers. Taken together, our results show that the TG-FLIM method enables high-throughput functional imaging of living C. elegans that can be used to study in vivo mechanisms of aggregation and that has the potential to aid the search for therapeutic modifiers of protein aggregation and toxicity.