PT  - JOURNAL ARTICLE
AU  - Laurent Gatto
AU  - Lisa M. Breckels
AU  - Kathryn S. Lilley
TI  - Assessing sub-cellular resolution in spatial proteomics experiments
AID  - 10.1101/377630
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 377630
4099  - http://biorxiv.org/content/early/2018/11/19/377630.short
4100  - http://biorxiv.org/content/early/2018/11/19/377630.full
AB  - The sub-cellular localisation of a protein is vital in defining its function, and a protein’s mis-localisation is known to lead to adverse effect. As a result, numerous experimental techniques and datasets have been published, with the aim of deciphering the localisation of proteins at various scales and resolutions, including high profile mass spectrometry-based efforts. Here, we present a meta-analysis assessing and comparing the sub-cellular resolution of 29 such mass spectrometry-based spatial proteomics experiments using a newly developed tool termed QSep. Our goal is to provide a simple quantitative report of how well spatial proteomics resolve the sub-cellular niches they describe to inform and guide developers and users of such methods.