RT Journal Article
SR Electronic
T1 The IncRNA <em>APOLO</em> interacts with the transcription factor WRKY42 to trigger root hair cell expansion in response to cold
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.07.13.188763
DO 10.1101/2020.07.13.188763
A1 Moison, Michaël
A1 Pacheco, Javier Martínez
A1 Lucero, Leandro
A1 Fonouni-Farde, Camille
A1 Rodríguez-Melo, Johan
A1 Christ, Aurélie
A1 Bazin, Jérémie
A1 Benhamed, Moussa
A1 Ibañez, Fernando
A1 Crespi, Martin
A1 Estevez, José M.
A1 Ariel, Federico
YR 2020
UL http://biorxiv.org/content/early/2020/07/14/2020.07.13.188763.abstract
AB Plant long noncoding RNAs (IncRNAs) have emerged as important regulators of chromatin dynamics, impacting on transcriptional programs leading to different developmental outputs. The lncRNA AUXIN REGULATED PROMOTER LOOP (APOLO) directly recognizes multiple independent loci across the Arabidopsis genome and modulates their three-dimensional chromatin conformation, leading to transcriptional shifts. Here, we show that APOLO recognizes the locus encoding the root hair (RH) master regulator ROOT HAIR DEFECTIVE 6 (RHD6) and controls RHD6 transcriptional activity leading to cold-enhanced RH elongation, in association with the Polycomb Repressive Complexes (PRC) 1 and 2. Additionally, we demonstrate that APOLO interacts with the transcription factor WRKY42 and modulates its binding to the RHD6 promoter. WRKY42 is required for the activation of RHD6 by low temperatures and WRKY42 deregulation impairs cold-induced RH expansion. Collectively, our results indicate that a novel ribonucleoprotein complex involving APOLO and WRKY42 forms a regulatory hub which activates RHD6 by shaping its epigenetic environment and integrates different signals governing RH growth and development.Competing Interest StatementThe authors have declared no competing interest.