PT  - JOURNAL ARTICLE
AU  - Xinhai Ye
AU  - Yi Yang
AU  - Zhaoyang Tian
AU  - Le Xu
AU  - Kaili Yu
AU  - Shan Xiao
AU  - Chuanlin Yin
AU  - Shijiao Xiong
AU  - Qi Fang
AU  - Hu Chen
AU  - Fei Li
AU  - Gongyin Ye
TI  - A high-quality <em>de novo</em> genome assembly from a single parasitoid wasp
AID  - 10.1101/2020.07.13.200725
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.07.13.200725
4099  - http://biorxiv.org/content/early/2020/07/14/2020.07.13.200725.short
4100  - http://biorxiv.org/content/early/2020/07/14/2020.07.13.200725.full
AB  - Sequencing and assembling a genome with a single individual have several advantages, such as lower heterozygosity and easier sample preparation. However, the amount of genomic DNA of some small sized organisms might not meet the standard DNA input requirement for current sequencing pipelines. Although few studies sequenced a single small insect with about 100 ng DNA as input, it may still be challenging for many small organisms to obtain such amount of DNA from a single individual. Here, we use 20 ng DNA as input, and present a high-quality genome assembly for a single haploid male parasitoid wasp (Habrobracon hebetor) using Nanopore and Illumina. Because of the low input DNA, a whole genome amplification (WGA) method is used before sequencing. The assembled genome size is 131.6 Mb with a contig N50 of 1.63 Mb. A total of 99% Benchmarking Universal Single-Copy Orthologs are detected, suggesting the high level of completeness of the genome assembly. Genome comparison between H. hebetor and its relative Bracon brevicornis shows a high-level genome synteny, indicating the genome of H. hebetor is highly accurate and contiguous. Our study provides an example for de novo assembling a genome from ultra-low input DNA, and will be used for sequencing projects of small sized species and rare samples, haploid genomics as well as population genetics of small sized species.Competing Interest StatementThe authors have declared no competing interest.