TY - JOUR T1 - Towards genetic modification of plant-parasitic nematodes: delivery of macromolecules to adults and expression of exogenous mRNA in second stage juveniles JF - bioRxiv DO - 10.1101/2020.07.15.193052 SP - 2020.07.15.193052 AU - Olaf Kranse AU - Helen Beasley AU - Sally Adams AU - Andre Pires da Silva AU - Chris Bell AU - Catherine Lilley AU - Peter Urwin AU - David Bird AU - Eric Miska AU - Geert Smant AU - Godelieve Gheysen AU - John Jones AU - Mark Viney AU - Pierre Abad AU - Thomas R. Maier AU - Thomas J. Baum AU - Shahid Siddique AU - Valerie Williamson AU - Alper Akay AU - Sebastian Eves-van den Akker Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/07/15/2020.07.15.193052.abstract N2 - Plant-parasitic nematodes are a continuing threat to food security, causing an estimated 100 billion USD in crop losses each year. The most problematic are the obligate sedentary endoparasites (primarily root knot nematodes and cyst nematodes). Progress in understanding their biology is held back by a lack of tools for functional genetics: forward genetics is largely restricted to studies of natural variation in populations, and reverse genetics is entirely reliant on RNA interference. There is an expectation that the development of functional genetic tools would accelerate the progress of research on plant-parasitic nematodes, and hence the development of novel control solutions. Here, we develop some of the foundational biology required to deliver a functional genetic tool kit in plant-parasitic nematodes. We characterise the gonads of male Heterodera schachtii and Meloidogyne hapla in the context of spermatogenesis. We test and optimise various methods for the delivery, expression, and/or detection of exogenous nucleic acids in plant-parasitic nematodes. We demonstrate that delivery of macromolecules to cyst and root knot nematode male germlines is difficult, but possible. Similarly, we demonstrate the delivery of oligonucleotides to root knot nematode gametes. Finally, we develop a transient expression system in plant-parasitic nematodes by demonstrating the delivery and expression of exogenous mRNA encoding various reporter genes throughout the body of H. schachtii juveniles using lipofectamine-based transfection. We anticipate these developments to be independently useful, will expedite the development of genetic modification tools for plant-parasitic nematodes, and ultimately catalyze research on a group of nematodes that threaten global food security.Competing Interest StatementThe authors have declared no competing interest. ER -